Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A Cohesin-RAD21 Interactome.

Panigrahi AK, Zhang N, Otta SK, Pati D

The cohesin complex holds the sister chromatids together from S phase until the metaphase-to-anaphase transition and ensures both their proper cohesin and timely separation. In addition to its canonical function in chromosomal segregation, cohesion has been suggested by several lines of investigation in recent years to play additional roles in apoptosis, DNA damage response, transcriptional regulation, and hematopoiesis. To better ... [more]

Unknown Dec. 07, 2011; 0(0); [Pubmed: 22145905]

Throughput

  • High Throughput

Ontology Terms

  • cell line: molt-4 cell (BTO:0000873) [acute t cell leukemia (DOID:5603)]

Curated By

  • BioGRID