An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Proteomics analysis of the interactome of N-myc downstream regulated gene 1 and its interactions with the androgen response program in prostate cancer cells.

Tu LC, Yan X, Hood L, Lin B

NDRG1 is known to play important roles in both androgen-induced cell differentiation and inhibition of prostate cancer metastasis. However, the proteins associated with NDRG1 function are not fully enumerated. Using coimmunoprecipitation and mass spectrometry analysis, we identified 58 proteins that interact with NDRG1 in prostate cancer cells. These proteins include nuclear proteins, adhesion molecules, endoplasmic reticulum (ER) chaperons, proteasome subunits, ... [more]

Mol. Cell Proteomics Apr. 01, 2007; 6(4);575-88 [Pubmed: 17220478]

Throughput

High Throughput

Ontology Terms

17beta-hydroxy-17-methylestra-4,9,11-trien-3-one (CHEBI:379896)
lncap cell (BTO:0001321)
prostate carcinoma (DOID:10286)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NDRG1 ACSL3	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Camara GA (2025)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

NDRG1

Gene Ontology Biological Process

Gene Ontology Molecular Function

Rab GTPase binding [IDA]cadherin binding [IDA]gamma-tubulin binding [IDA]microtubule binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

ACSL3

Gene Ontology Biological Process

Gene Ontology Molecular Function

long-chain fatty acid-CoA ligase activity [IMP]protein domain specific binding [IPI]protein kinase binding [IPI]