BAIT

HAI2

F22G5.22, F22G5_22, highly ABA-induced PP2C gene 2, AT1G07430
protein phosphatase 2C 3
Arabidopsis thaliana (Columbia)
PREY

RCAR3

MFH8.10, MFH8_10, PYL8, PYR1-like 8, regulatory components of ABA receptor 3, AT5G53160
regulatory component of ABA receptor 3
GO Process (3)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Functional roles of the protein phosphatase 2C, AtAIP1, in abscisic acid signaling and sugar tolerance in Arabidopsis.

Lim CW, Kim JH, Baek W, Kim BS, Lee SC

Biotic signaling molecules including abscisic acid (ABA) serve as an integrator of abiotic stress including high salinity and drought. Recent studies have led to the identification of an ABA signaling pathway from the ABA receptor to stomatal closure in response to abiotic stress. ABA is linked to ABA receptors and protein phosphatase 2C (PP2C) members. In this study, we reconstituted ... [more]

Plant Sci. May. 01, 2012; 187(0);83-8 [Pubmed: 22404835]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HAI2 RCAR3
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-BioGRID
-
HAI2 RCAR3
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
HAI2 RCAR3
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-
RCAR3 HAI2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID