BAIT

HAI2

F22G5.22, F22G5_22, highly ABA-induced PP2C gene 2, AT1G07430
protein phosphatase 2C 3
Arabidopsis thaliana (Columbia)
PREY

RCAR3

MFH8.10, MFH8_10, PYL8, PYR1-like 8, regulatory components of ABA receptor 3, AT5G53160
regulatory component of ABA receptor 3
GO Process (3)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Unique drought resistance functions of the Highly ABA-Induced Clade A protein phosphatase 2Cs.

Bhaskara GB, Nguyen TT, Verslues P

Six Arabidopsis Clade A protein phosphatase 2Cs (PP2Cs) have established ABA signaling roles; however, phenotypic roles of the remaining three "HAI" PP2Cs, Highly ABA-Induced1 (HAI1), AKT1-Interacting PP2C1/HAI2 (AIP1) and HAI3, have remained unclear. HAI PP2C mutants had enhanced proline and osmoregulatory solute accumulation at low water potential while mutants of other Clade A PP2Cs had no or lesser effect on ... [more]

Unknown Jul. 24, 2012; 0(0); [Pubmed: 22829320]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
HAI2 RCAR3
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-BioGRID
-
HAI2 RCAR3
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
RCAR3 HAI2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-
HAI2 RCAR3
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID