An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Arabidopsis GCP3-INTERACTING PROTEIN 1/MOZART1 is an integral component of the Î³-tubulin-containing microtubule nucleating complex.

Nakamura M, Yagi N, Kato T, Fujita S, Kawashima N, Ehrhardt DW, Hashimoto T

Microtubules in eukaryotic cells are nucleated from ring-shaped complexes that contain Î³-tubulin and a family of homologous Î³-tubulin complex proteins (GCPs), but the subunit composition of the complexes can vary among fungi, animals, and plants. Arabidopsis GCP3-Interacting Protein 1 (GIP1), a small protein with no homology to the GCP family, interacts with GCP3 in vitro, and is a plant homolog ... [more]

Unknown Mar. 10, 2012; 0(0); [Pubmed: 22404201]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
GCP2 GIP1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Nakamura M (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

GIP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

GCP2