BAIT

GCP2

ARABIDOPSIS THALIANA GAMMA-TUBULIN COMPLEX PROTEIN 2, ATGCP2, GAMMA-TUBULIN 2, MJJ3.10, MJJ3_10, TUBG2, gamma-tubulin complex protein 2, AT5G05620
tubulin gamma-2 chain
Arabidopsis thaliana (Columbia)
PREY

GIP1

ARABIDOPSIS ATGCP3 INTERACTING PROTEIN 1, ATGIP1, AtGCP3 interacting protein 1, T15G18.30, T15G18_30, AT4G09550
gamma-tubulin complex protein GIP1a
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Arabidopsis GCP3-INTERACTING PROTEIN 1/MOZART1 is an integral component of the γ-tubulin-containing microtubule nucleating complex.

Nakamura M, Yagi N, Kato T, Fujita S, Kawashima N, Ehrhardt DW, Hashimoto T

Microtubules in eukaryotic cells are nucleated from ring-shaped complexes that contain γ-tubulin and a family of homologous γ-tubulin complex proteins (GCPs), but the subunit composition of the complexes can vary among fungi, animals, and plants. Arabidopsis GCP3-Interacting Protein 1 (GIP1), a small protein with no homology to the GCP family, interacts with GCP3 in vitro, and is a plant homolog ... [more]

Unknown Mar. 10, 2012; 0(0); [Pubmed: 22404201]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
GIP1 GCP2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
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Curated By

  • BioGRID