BAIT

STE18

L000002122, YJR086W
G protein gamma subunit; forms a dimer with Ste4p to activate the mating signaling pathway, forms a heterotrimer with Gpa1p and Ste4p to dampen signaling; C-terminus is palmitoylated and farnesylated, which are required for normal signaling
GO Process (2)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

CYR1

CDC35, FIL1, HSR1, SRA4, TSM0185, adenylate cyclase, L000000467, YJL005W
Adenylate cyclase; required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
GO Process (2)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

[Roles of G-protein beta and gamma subunits in the interaction of G beta gamma with adenylyl cyclases II].

Li JJ, Lu N, Huang BR

To explore the individual roles of G protein beta and gamma subunit in the interactions with effectors.We investigated the interactions of G beta 1 and G beta 1 gamma 2 with adenylyl cyclase II(AC II) using the yeast two-hybrid and three-hybrid systems.When assayed for the abilities to activate the reporter gene, the interactions among AD-beta 1, gamma 2 and BD-AC ... [more]

Zhongguo Yi Xue Ke Xue Yuan Xue Bao Apr. 01, 2001; 23(2);115-8 [Pubmed: 12905884]

Throughput

  • Low Throughput

Additional Notes

  • interaction detected in three-hybrid system

Curated By

  • BioGRID