BAIT

NRD1

Nrd1 complex RNA-binding subunit, L000002862, YNL251C
RNA-binding subunit of Nrd1 complex; complex interacts with exosome to mediate 3'-end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with CTD of RNA pol II large subunit Rpo21p at phosphorylated Ser5 to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites; role in regulation of mitochondrial abundance and cell size
Saccharomyces cerevisiae (S288c)
PREY

SNF4

CAT3, SCI1, AMP-activated serine/threonine-protein kinase regulatory subunit SNF4, L000001947, YGL115W
Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress
Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

The S. cerevisiae Nrd1-Nab3 Transcription Termination Pathway Acts in Opposition to Ras Signaling and Mediates Response to Nutrient Depletion.

Darby MM, Serebreni L, Pan X, Boeke JD, Corden JL

The Saccharomyces cerevisiae Nrd1-Nab3 pathway directs termination and processing of short RNA polymerase II transcripts. Despite the potential for Nrd1-Nab3 to affect transcription of both coding and non-coding RNAs, little is known about how the Nrd1-Nab3 pathway interacts with other pathways in the cell. Here we present the results of a high-throughput synthetic lethality screen for genes that interact with ... [more]

Unknown Mar. 19, 2012; 0(0); [Pubmed: 22431520]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: vegetative growth (APO:0000106)

Additional Notes

  • dSLAM analysis performed on nrd1-102 mutant

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
NRD1 SNF4
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-3.4094BioGRID
221092

Curated By

  • BioGRID