NCOR1
Gene Ontology Biological Process
- Notch signaling pathway [TAS]
- cellular lipid metabolic process [TAS]
- gene expression [TAS]
- negative regulation of JNK cascade [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IMP, TAS]
- regulation of fatty acid transport [IC]
- regulation of glycolytic process by negative regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of lipid transport by negative regulation of transcription from RNA polymerase II promoter [IMP]
- small molecule metabolic process [TAS]
- spindle assembly [IMP]
- transcription from RNA polymerase II promoter [TAS]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PPARG
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [TAS]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- cell fate commitment [ISS]
- cell maturation [IDA]
- cellular response to insulin stimulus [IMP]
- epithelial cell differentiation [ISS]
- gene expression [TAS]
- glucose homeostasis [IMP]
- innate immune response [TAS]
- lipid homeostasis [TAS]
- lipid metabolic process [TAS]
- lipoprotein transport [IDA]
- long-chain fatty acid transport [ISS]
- low-density lipoprotein particle receptor biosynthetic process [IDA]
- monocyte differentiation [IDA]
- negative regulation of cholesterol storage [IDA]
- negative regulation of interferon-gamma-mediated signaling pathway [IMP]
- negative regulation of macrophage derived foam cell differentiation [IC, IDA]
- negative regulation of receptor biosynthetic process [IDA]
- negative regulation of sequestering of triglyceride [IDA]
- negative regulation of smooth muscle cell proliferation [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA, ISS]
- negative regulation of transcription, DNA-templated [ISS]
- peroxisome proliferator activated receptor signaling pathway [IMP]
- placenta development [ISS]
- positive regulation of fat cell differentiation [ISS]
- positive regulation of sequence-specific DNA binding transcription factor activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [ISS]
- regulation of blood pressure [IMP]
- regulation of cholesterol transporter activity [IC]
- regulation of transcription involved in cell fate commitment [ISS]
- response to lipid [ISS]
- response to low-density lipoprotein particle [IDA]
- response to nutrient [TAS]
- response to retinoic acid [IDA]
- signal transduction [IDA]
- transcription initiation from RNA polymerase II promoter [TAS]
- white fat cell differentiation [ISS, TAS]
Gene Ontology Molecular Function- DNA binding [IDA, ISS]
- activating transcription factor binding [IDA]
- arachidonic acid binding [ISS]
- chromatin binding [ISS]
- drug binding [IDA]
- enzyme binding [IPI]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- ligand-dependent nuclear receptor transcription coactivator activity [IDA]
- prostaglandin receptor activity [TAS]
- protein binding [IPI]
- retinoid X receptor binding [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISS]
- transcription regulatory region DNA binding [IDA, ISS]
- DNA binding [IDA, ISS]
- activating transcription factor binding [IDA]
- arachidonic acid binding [ISS]
- chromatin binding [ISS]
- drug binding [IDA]
- enzyme binding [IPI]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA]
- ligand-dependent nuclear receptor transcription coactivator activity [IDA]
- prostaglandin receptor activity [TAS]
- protein binding [IPI]
- retinoid X receptor binding [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA, ISS]
- transcription regulatory region DNA binding [IDA, ISS]
Gene Ontology Cellular Component
Affinity Capture-Luminescence
An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.
Publication
The endocrine disruptor monoethyl-hexyl-phthalate is a selective peroxisome proliferator-activated receptor gamma modulator that promotes adipogenesis.
The ability of pollutants to affect human health is a major concern, justified by the wide demonstration that reproductive functions are altered by endocrine disrupting chemicals. The definition of endocrine disruption is today extended to broader endocrine regulations, and includes activation of metabolic sensors, such as the peroxisome proliferator-activated receptors (PPARs). Toxicology approaches have demonstrated that phthalate plasticizers can directly ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 4.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PPARG NCOR1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PPARG NCOR1 | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | 504565 | |
| NCOR1 PPARG | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | 504567 | |
| PPARG NCOR1 | FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins. | Low | - | BioGRID | 658230 | |
| NCOR1 PPARG | Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments. | High | - | BioGRID | - | |
| PPARG NCOR1 | Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments. | Low | - | BioGRID | - | |
| PPARG NCOR1 | Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments. | Low | - | BioGRID | - | |
| PPARG NCOR1 | Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments. | Low | - | BioGRID | - | |
| PPARG NCOR1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3318155 | |
| NCOR1 PPARG | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| PPARG NCOR1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| PPARG NCOR1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| PPARG NCOR1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| NCOR1 PPARG | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| PPARG NCOR1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | 2596027 | |
| NCOR1 PPARG | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID