BAIT
E2F1
E2F-1, RBAP1, RBBP3, RBP3
E2F transcription factor 1
GO Process (18)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
- DNA damage checkpoint [IMP]
- G1/S transition of mitotic cell cycle [TAS]
- Notch signaling pathway [TAS]
- apoptotic process [TAS]
- intrinsic apoptotic signaling pathway [TAS]
- intrinsic apoptotic signaling pathway in response to DNA damage [IMP]
- mRNA stabilization [IDA]
- mitotic cell cycle [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription involved in G1/S transition of mitotic cell cycle [IMP]
- negative regulation of transcription, DNA-templated [IMP]
- positive regulation of fibroblast proliferation [IMP]
- positive regulation of gene expression [IDA]
- positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [TAS]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription, DNA-templated [IMP]
- regulation of G1/S transition of mitotic cell cycle [IMP]
- regulation of transcription, DNA-templated [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
IGF1
IGF-I, IGF1A, IGFI
insulin-like growth factor 1 (somatomedin C)
GO Process (50)
GO Function (5)
GO Component (5)
Gene Ontology Biological Process
- DNA replication [TAS]
- Ras protein signal transduction [TAS]
- blood coagulation [TAS]
- bone mineralization involved in bone maturation [IDA]
- cell activation [IDA]
- cellular component movement [TAS]
- cellular protein metabolic process [TAS]
- glycolate metabolic process [TAS]
- muscle hypertrophy [IMP]
- muscle organ development [TAS]
- myoblast differentiation [IDA]
- myoblast proliferation [IDA]
- myotube cell development [IDA]
- negative regulation of extrinsic apoptotic signaling pathway [IDA]
- negative regulation of oocyte development [IMP]
- negative regulation of release of cytochrome c from mitochondria [ISS]
- negative regulation of smooth muscle cell apoptotic process [IDA]
- phosphatidylinositol-mediated signaling [IDA]
- platelet activation [TAS]
- platelet degranulation [TAS]
- positive regulation of DNA binding [IDA]
- positive regulation of DNA replication [IDA, ISS]
- positive regulation of MAPK cascade [IDA]
- positive regulation of Ras protein signal transduction [IDA]
- positive regulation of activated T cell proliferation [IDA]
- positive regulation of calcineurin-NFAT signaling cascade [IDA]
- positive regulation of cardiac muscle hypertrophy [IDA]
- positive regulation of cell proliferation [IDA]
- positive regulation of epithelial cell proliferation [IDA]
- positive regulation of fibroblast proliferation [IDA]
- positive regulation of glucose import [IDA]
- positive regulation of glycogen biosynthetic process [IDA]
- positive regulation of glycolytic process [IDA]
- positive regulation of insulin-like growth factor receptor signaling pathway [IDA]
- positive regulation of mitosis [IDA]
- positive regulation of osteoblast differentiation [IDA]
- positive regulation of peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of phosphatidylinositol 3-kinase signaling [IDA]
- positive regulation of protein import into nucleus, translocation [IDA]
- positive regulation of smooth muscle cell migration [IDA]
- positive regulation of smooth muscle cell proliferation [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [IDA]
- positive regulation of tyrosine phosphorylation of Stat5 protein [IDA]
- proteoglycan biosynthetic process [IDA]
- regulation of gene expression [IMP]
- regulation of multicellular organism growth [IEP]
- signal transduction [TAS]
- skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration [IDA]
- skeletal system development [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Tumor necrosis factor alpha inhibits cyclin A expression and retinoblastoma hyperphosphorylation triggered by insulin-like growth factor-I induction of new E2F-1 synthesis.
Cyclin A is required for cell cycle S phase entry, and its overexpression contributes to tumorigenesis. Release of pre-existing E2Fs from inactive complexes of E2F and hypophosphorylated retinoblastoma (RB) is the prevailing dogma for E2F transcriptional activation of target genes such as cyclin A. Here we explored the hypothesis that new synthesis of E2F-1 is required for insulin-like growth factor-I ... [more]
J. Biol. Chem. Feb. 27, 2004; 279(9);7438-46 [Pubmed: 14681231]
Throughput
- Low Throughput
Additional Notes
- figure 7. E2F-1 mediated cyclin A expression was enhanced by IGF-I, and inhibited by TNF.
Curated By
- BioGRID