BAIT

DMA1

CHF1, ubiquitin-conjugating protein DMA1, S000029719, YHR115C
Ubiquitin-protein ligase (E3); controls septin dynamics, spindle position checkpoint (SPOC) with ligase Dma2p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8, similar to human Chfr; contains FHA, RING fingers; DMA1 has a paralog, DMA2, that arose from the whole genome duplication
UPS Project
Saccharomyces cerevisiae (S288c)
PREY

ELM1

LDB9, serine/threonine protein kinase ELM1, L000000548, YKL048C
Serine/threonine protein kinase that regulates cellular morphogenesis; septin behavior, and cytokinesis; required for the regulation of other kinases, such as Kin4p; forms part of the bud neck ring
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)

Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Publication

Budding yeast dma proteins control septin dynamics and the spindle position checkpoint by promoting the recruitment of the elm1 kinase to the bud neck.

Merlini L, Fraschini R, Boettcher B, Barral Y, Lucchini G, Piatti S

The first step towards cytokinesis in budding yeast is the assembly of a septin ring at the future site of bud emergence. Integrity of this ring is crucial for cytokinesis, proper spindle positioning, and the spindle position checkpoint (SPOC). This checkpoint delays mitotic exit and cytokinesis as long as the anaphase spindle does not properly align with the division axis. ... [more]

PLoS Genet. Apr. 01, 2012; 8(4);e1002670 [Pubmed: 22570619]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: position of spindle pole body (APO:0000214)
  • phenotype: viability (APO:0000111)

Additional Notes

  • an elm1-bni4 chimeric protein can suppress lethality and septin defects caused by mutation of a dma1/dma2 mutant
  • genetic complex

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ELM1 DMA1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
659084

Curated By

  • BioGRID