BAIT

ELAVL1

ELAV1, HUR, Hua, MelG
ELAV like RNA binding protein 1
Homo sapiens
PREY

ARHGEF11

GTRAP48, PDZ-RHOGEF, RP11-356J7.2
Rho guanine nucleotide exchange factor (GEF) 11
Cell Cycle - Mitotic Spindle Assembly Project
Homo sapiens

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Ubiquitin-mediated proteolysis of HuR by heat shock.

Abdelmohsen K, Srikantan S, Yang X, Lal A, Kim HH, Kuwano Y, Galban S, Becker KG, Kamara D, de Cabo R, Gorospe M

The RNA-binding protein HuR regulates the stability and translation of numerous mRNAs encoding stress-response and proliferative proteins. Although its post-transcriptional influence has been linked primarily to its cytoplasmic translocation, here we report that moderate heat shock (HS) potently reduces HuR levels, thereby altering the expression of HuR target mRNAs. HS did not change HuR mRNA levels or de novo translation, ... [more]

EMBO J. May. 06, 2009; 28(9);1271-82 [Pubmed: 19322201]

Throughput

  • High Throughput

Ontology Terms

  • hela cell (BTO:0000567) [cervical adenocarcinoma (DOID:3702)]

Curated By

  • BioGRID