Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

RING finger protein 149 is an E3 ubiquitin ligase active on wild-type BRAF.

Hong SW, Jin DH, Shin JS, Moon JH, Na YS, Jung KA, Kim SM, Kim JC, Kim KP, Hong YS, Lee JL, Choi EK, Lee JS, Kim TW

Members of the Raf family (ARAF, BRAF, and CRAF/Raf-1) are involved in a variety of cellular activities, including growth, survival,differentiation, and transformation. An oncogene encodes BRAF, the function of which is linked to MEK activation. BRAF is the most effective Raf kinase in terms of induction of MEK/ERK activity. However, the mechanisms involved in BRAF regulation remain unclear. In the ... [more]

Unknown May. 24, 2012; 0(0); [Pubmed: 22628551]

Throughput

  • Low Throughput

Curated By

  • BioGRID