ALP14
Gene Ontology Biological Process
- attachment of mitotic spindle microtubules to kinetochore [IDA]
- cytoplasmic microtubule organization [IMP]
- establishment or maintenance of cell polarity regulating cell shape [IMP]
- microtubule cytoskeleton organization [IMP]
- mitotic sister chromatid segregation [IMP]
- spindle assembly involved in mitosis [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
NDA3
Gene Ontology Biological Process
Gene Ontology Cellular Component
Biochemical Activity
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
Fission yeast Alp14 is a dose dependent plus end tracking microtubule polymerase.
XMAP215/Dis1 proteins are conserved tubulin-binding TOG domain proteins that regulate microtubule (MT) plus end dynamics. Here, we show that Alp14, a XMAP215 orthologue in fission yeast Schizosaccharomyces pombe, has properties of a MT polymerase. In vivo, Alp14 localizes to growing MT plus ends, in a manner independent of Mal3 (EB1). alp14 null mutants display short interphase MTs with twofold slower ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 3. microtubule polymerase.
- figure 4. Alp14-GFP tracks growing MT plus ends in vitro.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ALP14 NDA3 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | 664502 | |
| ALP14 NDA3 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | Low | - | BioGRID | 246772 | |
| ALP14 NDA3 | Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene. | Low | - | PomBase | - |
Curated By
- BioGRID