ALP14
Gene Ontology Biological Process
- attachment of mitotic spindle microtubules to kinetochore [IDA]
- cytoplasmic microtubule organization [IMP]
- establishment or maintenance of cell polarity regulating cell shape [IMP]
- microtubule cytoskeleton organization [IMP]
- mitotic sister chromatid segregation [IMP]
- spindle assembly involved in mitosis [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
NDA3
Gene Ontology Biological Process
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is detected between purified proteins in vitro.
Publication
Fission yeast Alp14 is a dose dependent plus end tracking microtubule polymerase.
XMAP215/Dis1 proteins are conserved tubulin-binding TOG domain proteins that regulate microtubule (MT) plus end dynamics. Here, we show that Alp14, a XMAP215 orthologue in fission yeast Schizosaccharomyces pombe, has properties of a MT polymerase. In vivo, Alp14 localizes to growing MT plus ends, in a manner independent of Mal3 (EB1). alp14 null mutants display short interphase MTs with twofold slower ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 5. TOG domain mutant on Alp14 abolishes its ability for tubulin binding.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ALP14 NDA3 | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 664500 | |
| ALP14 NDA3 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | Low | - | BioGRID | 246772 | |
| ALP14 NDA3 | Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene. | Low | - | PomBase | - |
Curated By
- BioGRID