BAIT
RAD23A
HHR23A, HR23A
RAD23 homolog A (S. cerevisiae)
GO Process (3)
GO Function (4)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
BASP1
CAP-23, CAP23, NAP-22, NAP22
brain abundant, membrane attached signal protein 1
GO Process (10)
GO Function (4)
GO Component (9)
Gene Ontology Biological Process
- diaphragm development [ISS]
- glomerular visceral epithelial cell differentiation [ISS]
- gonad development [ISS]
- mesenchymal to epithelial transition [ISS]
- metanephric mesenchyme development [ISS]
- negative regulation of transcription, DNA-templated [IDA, IMP]
- positive regulation of heart growth [ISS]
- positive regulation of metanephric ureteric bud development [ISS]
- substantia nigra development [IEP]
- thorax and anterior abdomen determination [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Isolation of human proteasomes and putative proteasome-interacting proteins using a novel affinity chromatography method.
The proteasome is the primary subcellular organelle responsible for protein degradation. It is a dynamic assemblage of 34 core subunits and many differentially expressed, transiently interacting, modulatory proteins. This paper describes a novel affinity chromatography method for the purification of functional human holoproteasome complexes using mild conditions. Human proteasomes purified by this simple procedure maintained the ability to proteolytically process ... [more]
Exp. Cell Res. Jan. 15, 2009; 315(2);176-89 [Pubmed: 19013454]
Throughput
- Low Throughput
Curated By
- BioGRID