BAIT

KAR3

OSR11, L000000888, YPR141C
Minus-end-directed microtubule motor; functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate
Saccharomyces cerevisiae (S288c)
PREY

TUB2

ARM10, SHE8, beta-tubulin, L000002388, YFL037W
Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
Saccharomyces cerevisiae (S288c)

Co-crystal Structure

Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.

Publication

Kar3Vik1, a member of the Kinesin-14 superfamily, shows a novel kinesin microtubule binding pattern.

Rank KC, Chen CJ, Cope J, Porche K, Hoenger A, Gilbert SP, Rayment I

Kinesin-14 motors generate microtubule minus-end-directed force used in mitosis and meiosis. These motors are dimeric and operate with a nonprocessive powerstroke mechanism, but the role of the second head in motility has been unclear. In Saccharomyces cerevisiae, the Kinesin-14 Kar3 forms a heterodimer with either Vik1 or Cik1. Vik1 contains a motor homology domain that retains microtubule binding properties but ... [more]

J. Cell Biol. Jun. 25, 2012; 197(7);957-70 [Pubmed: 22734002]

Throughput

  • Low Throughput

Additional Notes

  • interaction viewed by EM

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TUB2 KAR3
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.3644BioGRID
1979150
KAR3 TUB2
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
KAR3 TUB2
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-
TUB2 KAR3
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
470636

Curated By

  • BioGRID