An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

H2A.Z.2.2 is an alternatively spliced histone H2A.Z variant that causes severe nucleosome destabilization.

Boenisch C, Schneider K, Puenzeler S, Wiedemann SM, Bielmeier C, Bocola M, Eberl HC, Kuegel W, Neumann J, Kremmer E, Leonhardt H, Mann M, Michaelis J, Schermelleh L, Hake SB

The histone variant H2A.Z has been implicated in many biological processes, such as gene regulation and genome stability. Here, we present the identification of H2A.Z.2.2 (Z.2.2), a novel alternatively spliced variant of histone H2A.Z and provide a comprehensive characterization of its expression and chromatin incorporation properties. Z.2.2 mRNA is found in all human cell lines and tissues with highest levels ... [more]

Unknown Mar. 29, 2012; 0(0); [Pubmed: 22467210]

Throughput

High Throughput

Additional Notes

figure 4A. Also for H2A.Z.2.2: non-specific interactors filtered out.
figure 4A. For H2A.Z.2.1: non-specific interactors filtered out.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
H2AFZ ACTL6A	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Cai Y (2007)	Low	-	BioGRID	-
H2AFZ ACTL6A	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Cho NH (2022)	High	-	BioGRID	3357175
H2AFZ ACTL6A	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9673	BioGRID	3112046

Curated By

BioGRID

Interaction Summary

H2AFZ

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]RNA polymerase II core promoter sequence-specific DNA binding [IDA]RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]chromatin DNA binding [IDA]nucleosomal DNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

ACTL6A