DOT1L
Gene Ontology Biological Process
Gene Ontology Molecular Function
BAG6
Gene Ontology Biological Process
- brain development [ISS]
- embryo development [ISS]
- internal peptidyl-lysine acetylation [IDA]
- intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IMP]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [ISS]
- kidney development [ISS]
- lung development [ISS]
- negative regulation of proteasomal ubiquitin-dependent protein catabolic process [ISS]
- negative regulation of proteolysis [ISS]
- protein stabilization [ISS]
- spermatogenesis [ISS]
- synaptonemal complex assembly [ISS]
- tail-anchored membrane protein insertion into ER membrane [IDA]
- ubiquitin-dependent protein catabolic process [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Bat3 facilitates H3K79 dimethylation by DOT1L and promotes DNA damage-induced 53BP1 foci at G1/G2 cell-cycle phases.
The methyltransferase DOT1L methylates histone H3 at K79 to facilitate specific biological events. H3K79 dimethylation (H3K79-2Me) by DOT1L influences the DNA damage response by promoting 53BP1 recruitment to DNA damage sites; however, it is unclear if this methylation is required as 53BP1 interacts with dimethylated H4 (H4K20-2Me) with a much higher affinity. We demonstrate that H3K79-2Me, while negligible during S-phase, ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 4A.
- figure 6. A novel DOT1L UIM is required for DOT1L-Bat3 interaction and efficient H3K79-2Me and 53BP1 foci formation
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| BAG6 DOT1L | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 670304 |
Curated By
- BioGRID