A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Screening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry.

Berendzen KW, Boehmer M, Wallmeroth N, Peter S, Vesi 263 M, Zhou Y, Tiesler FK, Schleifenbaum F, Harter K

ABSTRACT: Understanding protein and gene function requires identifying interaction partners usingbiochemical, molecular or genetic tools. In plants, searching for novel protein-proteininteractions is limited to protein purification assays, heterologous in vivo systems such as the yeast-two-hybrid or mutant screens. Ideally one would be able to search for novel proteinpartners in living plant cells. We demonstrate that it is possible to screen ... [more]

Unknown Jul. 12, 2012; 8(1);25 [Pubmed: 22789293]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CDPK6 APX3	FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.	Berendzen KW (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CDPK6

Gene Ontology Biological Process

Gene Ontology Molecular Function

calmodulin-dependent protein kinase activity [ISS]kinase activity [ISS]protein binding [IPI]protein kinase activity [IDA]protein serine/threonine kinase activity [IDA]

Gene Ontology Cellular Component

APX3