An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Screening for in planta protein-protein interactions combining bimolecular fluorescence complementation with flow cytometry.

Berendzen KW, Boehmer M, Wallmeroth N, Peter S, Vesi 263 M, Zhou Y, Tiesler FK, Schleifenbaum F, Harter K

ABSTRACT: Understanding protein and gene function requires identifying interaction partners usingbiochemical, molecular or genetic tools. In plants, searching for novel protein-proteininteractions is limited to protein purification assays, heterologous in vivo systems such as the yeast-two-hybrid or mutant screens. Ideally one would be able to search for novel proteinpartners in living plant cells. We demonstrate that it is possible to screen ... [more]

Unknown Jul. 12, 2012; 8(1);25 [Pubmed: 22789293]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CDPK6 APX3	PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.	Berendzen KW (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CDPK6

Gene Ontology Biological Process

Gene Ontology Molecular Function

calmodulin-dependent protein kinase activity [ISS]kinase activity [ISS]protein binding [IPI]protein kinase activity [IDA]protein serine/threonine kinase activity [IDA]

Gene Ontology Cellular Component

APX3