BAIT

ITGB3

BDPLT16, BDPLT2, CD61, GP3A, GPIIIa, GT
integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
GO Process (36)
GO Function (9)
GO Component (16)
Homo sapiens
PREY

SYK

p72-Syk
spleen tyrosine kinase
GO Process (38)
GO Function (6)
GO Component (8)

Gene Ontology Biological Process

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Platelet alpha IIb-beta 3 integrin engagement induces the tyrosine phosphorylation of Cbl and its association with phosphoinositide 3-kinase and Syk.

Saci A, Rendu F, Bachelot-Loza C

Agonist-induced platelet activation triggers 'inside-out' signalling which activates alpha IIb-beta 3, the most abundant integrin in platelet membranes. The engagement of activated alpha IIb-beta 3 integrin by linking fibrinogen is necessary for platelet aggregation, and this induces subsequent outside-in signalling, which enhances platelet activation. Here we studied the involvement of Cbl during alpha IIb-beta 3-integrin-mediated signal transduction. During thrombin-induced platelet ... [more]

Biochem. J. Nov. 01, 2000; 351(0);669-76 [Pubmed: 11042121]

Throughput

  • Low Throughput

Curated By

  • BioGRID