An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The serine/arginine-rich protein SF2/ASF regulates protein sumoylation.

Pelisch F, Gerez J, Druker J, Schor IE, Munoz MJ, Risso G, Petrillo E, Westman BJ, Lamond AI, Arzt E, Srebrow A

Protein modification by conjugation of small ubiquitin-related modifier (SUMO) is involved in diverse biological functions, such as transcription regulation, subcellular partitioning, stress response, DNA damage repair, and chromatin remodeling. Here, we show that the serine/arginine-rich protein SF2/ASF, a factor involved in splicing regulation and other RNA metabolism-related processes, is a regulator of the sumoylation pathway. The overexpression of this protein ... [more]

Proc. Natl. Acad. Sci. U.S.A. Sep. 14, 2010; 107(37);16119-24 [Pubmed: 20805487]

Throughput

Low Throughput

Additional Notes

figure 2A.
figure S7A.

Curated By

BioGRID

Interaction Summary

UBE2I

Gene Ontology Biological Process

Gene Ontology Molecular Function

RING-like zinc finger domain binding [IPI]SUMO transferase activity [IDA]enzyme binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]transcription factor binding [IPI]ubiquitin protein ligase activity [IBA]ubiquitin protein ligase binding [IBA]ubiquitin-protein transferase activity [TAS]

Gene Ontology Cellular Component

SRSF1