Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

FBG1 is a promiscuous ubiquitin ligase that sequesters APC2 and causes S-phase arrest.

Wen H, Kim N, Fuentes EJ, Mallinger A, Gonzalez-Alegre P, Glenn K

During cell proliferation, protein degradation is strictly regulated by the cell cycle and involves two complementary ubiquitin ligase complexes, the SCF (Skp, Cullin, F-box) and APC/C (Anaphase Promoting Complex/Cyclosome) ubiquitin ligases. SCF ligases are constitutively active and generally target only proteins after they have been selected for degradation, usually by phosphorylation. In contrast, APC/C complexes are themselves activated by phosphorylation ... [more]

Cell Cycle Nov. 15, 2010; 9(22);4506-17 [Pubmed: 21135578]

Throughput

  • Low Throughput

Curated By

  • BioGRID