BAIT
HSP90AA1
EL52, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP-2, LAP2
heat shock protein 90kDa alpha (cytosolic), class A member 1
GO Process (16)
GO Function (10)
GO Component (10)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- G2/M transition of mitotic cell cycle [TAS]
- axon guidance [TAS]
- chaperone-mediated protein complex assembly [IDA]
- innate immune response [TAS]
- mitochondrial transport [TAS]
- mitotic cell cycle [TAS]
- nitric oxide metabolic process [TAS]
- positive regulation of nitric oxide biosynthetic process [ISS]
- protein import into mitochondrial outer membrane [IDA]
- protein refolding [TAS]
- regulation of nitric-oxide synthase activity [TAS]
- response to unfolded protein [NAS]
- signal transduction [NAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PPARA
PPAR
peroxisome proliferator activated receptor alpha
GO Process (33)
GO Function (20)
GO Component (1)
Gene Ontology Biological Process
- behavioral response to nicotine [IDA]
- circadian regulation of gene expression [ISO, ISS]
- enamel mineralization [ISO]
- epidermis development [ISO]
- fatty acid metabolic process [IMP, ISO]
- heart development [IEP]
- intracellular receptor signaling pathway [IMP, ISO]
- lipoprotein metabolic process [IMP]
- negative regulation of appetite [ISO, ISS]
- negative regulation of blood pressure [IMP]
- negative regulation of cholesterol storage [ISO]
- negative regulation of macrophage derived foam cell differentiation [ISO]
- negative regulation of neuron death [IDA, IMP]
- negative regulation of protein binding [IMP]
- negative regulation of receptor biosynthetic process [ISO]
- negative regulation of sequestering of triglyceride [ISO]
- negative regulation of transcription from RNA polymerase II promoter [ISO]
- negative regulation of transcription regulatory region DNA binding [IMP]
- positive regulation of fatty acid oxidation [ISO]
- positive regulation of gluconeogenesis [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of transcription, DNA-templated [ISO, ISS]
- regulation of cellular ketone metabolic process by positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of circadian rhythm [ISO, ISS]
- regulation of fatty acid metabolic process [ISO, TAS]
- regulation of gene expression [ISO]
- regulation of glycolytic by positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of lipid transport by positive regulation of transcription from RNA polymerase II promoter [ISO]
- regulation of transcription, DNA-templated [IDA]
- response to hypoxia [IDA]
- response to insulin [IMP]
- transcription from RNA polymerase II promoter [ISO]
- wound healing [ISO]
Gene Ontology Molecular Function- DNA binding [ISO]
- MDM2/MDM4 family protein binding [IPI]
- NFAT protein binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- RNA polymerase II repressing transcription factor binding [ISO]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [ISO]
- drug binding [ISO, ISS]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IMP, ISO]
- lipid binding [ISO, ISS]
- phosphatase binding [IPI]
- protein binding [IPI]
- protein complex binding [IMP, IPI]
- protein domain specific binding [IDA]
- receptor activity [ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [ISO]
- steroid hormone receptor activity [ISO, TAS]
- ubiquitin conjugating enzyme binding [ISO]
- DNA binding [ISO]
- MDM2/MDM4 family protein binding [IPI]
- NFAT protein binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- RNA polymerase II repressing transcription factor binding [ISO]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [ISO]
- drug binding [ISO, ISS]
- ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IMP, ISO]
- lipid binding [ISO, ISS]
- phosphatase binding [IPI]
- protein binding [IPI]
- protein complex binding [IMP, IPI]
- protein domain specific binding [IDA]
- receptor activity [ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [ISO]
- steroid hormone receptor activity [ISO, TAS]
- ubiquitin conjugating enzyme binding [ISO]
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Heat shock protein-90 (Hsp90) acts as a repressor of peroxisome proliferator-activated receptor-alpha (PPARalpha) and PPARbeta activity.
The nuclear receptor (NR) peroxisome proliferator-activated receptor-alpha (PPARalpha) mediates the effects of several hypolipidemic drugs, endogenous fatty acids, and peroxisome proliferators. Despite belonging to a class of NR not known to interact with cytosolic chaperone complexes, we have recently shown that PPARalpha interacts with heat shock protein 90 (Hsp90), although the biological consequence of this association was unknown. In the ... [more]
Biochemistry Sep. 16, 2003; 42(36);10726-35 [Pubmed: 12962497]
Throughput
- Low Throughput
Curated By
- BioGRID