BAIT
TFAP4
AP-4, bHLHc41
transcription factor AP-4 (activating enhancer binding protein 4)
GO Process (13)
GO Function (10)
GO Component (2)
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator [IDA]
- negative regulation by host of viral transcription [IDA, NAS]
- negative regulation of DNA binding [IDA]
- negative regulation of cell cycle arrest [IMP]
- negative regulation of cell proliferation [IMP]
- negative regulation of cyclin-dependent protein serine/threonine kinase activity [IDA]
- negative regulation of transcription, DNA-templated [IDA, IMP]
- positive regulation by host of viral transcription [IDA]
- positive regulation of apoptotic process [IDA]
- positive regulation of transcription, DNA-templated [IMP]
- protein complex assembly [IDA]
- regulation of transcription from RNA polymerase II promoter [IBA]
- transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function- DNA binding [IDA]
- E-box binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IMP]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- transcription coactivator activity [IDA]
- transcription regulatory region DNA binding [IDA]
- DNA binding [IDA]
- E-box binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IMP]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- transcription coactivator activity [IDA]
- transcription regulatory region DNA binding [IDA]
Gene Ontology Cellular Component
Homo sapiens
PREY
CKAP5
CHTOG, MSPS, TOG, TOGp, ch-TOG
cytoskeleton associated protein 5
GO Process (6)
GO Function (1)
GO Component (8)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Complementary quantitative proteomics reveals that transcription factor AP-4 mediates E-box-dependent complex formation for transcriptional repression of HDM2.
Transcription factor activating enhancer-binding protein 4 (AP-4) is a basic helix-loop-helix protein that binds to E-box elements. AP-4 has received increasing attention for its regulatory role in cell growth and development, including transcriptional repression of the human homolog of murine double minute 2 (HDM2), an important oncoprotein controlling cell growth and survival, by an unknown mechanism. Here we demonstrate that ... [more]
Mol. Cell Proteomics Sep. 01, 2009; 8(9);2034-50 [Pubmed: 19505873]
Throughput
- High Throughput
Additional Notes
- table 1. potential AP-4-interacting proteins differentially bound to the HDM2-P2 promoter E-box sequence.
Curated By
- BioGRID