BAIT
CUL3
CUL-3, PHA2E
cullin 3
GO Process (20)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- COPII vesicle coating [IMP]
- ER to Golgi vesicle-mediated transport [IDA]
- G1/S transition of mitotic cell cycle [TAS]
- cell cycle arrest [TAS]
- cell migration [IMP]
- embryonic cleavage [ISS]
- integrin-mediated signaling pathway [ISS]
- intrinsic apoptotic signaling pathway [TAS]
- mitotic metaphase plate congression [IMP]
- negative regulation of Rho protein signal transduction [IMP]
- negative regulation of cyclin-dependent protein serine/threonine kinase by cyclin degradation [IDA]
- positive regulation of cell proliferation [TAS]
- positive regulation of cytokinesis [IMP]
- positive regulation of mitotic metaphase/anaphase transition [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein monoubiquitination [IDA]
- protein polyubiquitination [IDA]
- protein ubiquitination [IDA]
- stem cell division [ISS]
- stress fiber assembly [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PPP1CB
HEL-S-80p, PP-1B, PP1B, PP1beta, PPP1CD
protein phosphatase 1, catalytic subunit, beta isozyme
GO Process (11)
GO Function (5)
GO Component (6)
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- circadian regulation of gene expression [ISS]
- entrainment of circadian clock by photoperiod [ISS]
- mitotic cell cycle [TAS]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- protein dephosphorylation [ISS]
- regulation of cell adhesion [IDA]
- regulation of circadian rhythm [IMP]
- small molecule metabolic process [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
- triglyceride catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics.
Dynamic reorganization of signaling systems frequently accompanies pathway perturbations, yet quantitative studies of network remodeling by pathway stimuli are lacking. Here, we report the development of a quantitative proteomics platform centered on multiplex absolute quantification (AQUA) technology to elucidate the architecture of the cullin-RING ubiquitin ligase (CRL) network and to evaluate current models of dynamic CRL remodeling. Current models suggest ... [more]
Cell Dec. 10, 2010; 143(6);951-65 [Pubmed: 21145461]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293t cell (BTO:0002181)
Additional Notes
- All data was filtered to a 1% false discovery rate (peptide level) prior to analysis using CompPASS to identify high confidence candidate interacting proteins
- TAP-tagged Cul3
- exogenous expression of bait
Curated By
- BioGRID