Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease.

Goehler H, Lalowski M, Stelzl U, Waelter S, Stroedicke M, Worm U, Droege A, Lindenberg KS, Knoblich M, Haenig C, Herbst M, Suopanki J, Scherzinger E, Abraham C, Bauer B, Hasenbank R, Fritzsche A, Ludewig AH, Buessow K, Buessow K, Coleman SH, Gutekunst CA, Landwehrmeyer BG, Lehrach H, Wanker EE

Analysis of protein-protein interactions (PPIs) is a valuable approach for characterizing proteins of unknown function. Here, we have developed a strategy combining library and matrix yeast two-hybrid screens to generate a highly connected PPI network for Huntington's disease (HD). The network contains 186 PPIs among 35 bait and 51 prey proteins. It revealed 165 new potential interactions, 32 of which ... [more]

Mol. Cell Sep. 24, 2004; 15(6);853-65 [Pubmed: 15383276]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FEZ1 HTT	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2017)	High	0.9747	BioGRID	2246515
FEZ1 HTT	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Goehler H (2004)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

HTT

Gene Ontology Biological Process

Gene Ontology Molecular Function

beta-tubulin binding [IDA]dynactin binding [IPI]dynein intermediate chain binding [IDA]identical protein binding [IPI]ion channel binding [IDA]p53 binding [IPI]protein binding [IPI]transcription factor binding [IBA]

Gene Ontology Cellular Component

FEZ1