Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

ESCRT-dependent targeting of plasma membrane localized KCa3.1 to the lysosomes.

Balut CM, Gao Y, Murray SA, Thibodeau PH, Devor DC

The number of intermediate-conductance, Ca(2+)-activated K(+) channels (KCa3.1) present at the plasma membrane is deterministic in any physiological response. However, the mechanisms by which KCa3.1 channels are removed from the plasma membrane and targeted for degradation are poorly understood. Recently, we demonstrated that KCa3.1 is rapidly internalized from the plasma membrane, having a short half-life in both human embryonic kidney ... [more]

Am. J. Physiol., Cell Physiol. Nov. 01, 2010; 299(5);C1015-27 [Pubmed: 20720181]

Throughput

  • Low Throughput

Curated By

  • BioGRID