NFATC1
Gene Ontology Biological Process
Gene Ontology Molecular Function- FK506 binding [TAS]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISS]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [ISS]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISS]
- RNA polymerase II transcription factor binding [ISS]
- mitogen-activated protein kinase p38 binding [ISS]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [TAS]
- transcription regulatory region DNA binding [IDA]
- FK506 binding [TAS]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISS]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [ISS]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISS]
- RNA polymerase II transcription factor binding [ISS]
- mitogen-activated protein kinase p38 binding [ISS]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [TAS]
- transcription regulatory region DNA binding [IDA]
Gene Ontology Cellular Component
RPS6KB1
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IMP]
- TOR signaling [IDA]
- cellular response to growth factor stimulus [IDA]
- insulin receptor signaling pathway [TAS]
- negative regulation of apoptotic process [IMP]
- negative regulation of insulin receptor signaling pathway [IMP]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of mitotic cell cycle [IMP]
- positive regulation of translation [IMP]
- positive regulation of translational initiation [IMP]
- protein phosphorylation [IDA]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Elucidating protein: DNA complex by oligonucleotide DNA affinity purification.
Transcription factors recruit a wide variety of associated co-factors to regulate gene expression. These co-factors include protein kinases, phosphatases, deacetylases, methylases, and ubiquitin ligases, etc. To identify novel protein kinases associated with transcription factor NFAT, we took advantage of the increased ability of DNA binding and used an oligonucleotide affinity-binding approach. Coupling with in-gel kinase assays to detect phosphotransferase activity, ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID