CUL3
Gene Ontology Biological Process
- COPII vesicle coating [IMP]
- ER to Golgi vesicle-mediated transport [IDA]
- G1/S transition of mitotic cell cycle [TAS]
- cell cycle arrest [TAS]
- cell migration [IMP]
- embryonic cleavage [ISS]
- integrin-mediated signaling pathway [ISS]
- intrinsic apoptotic signaling pathway [TAS]
- mitotic metaphase plate congression [IMP]
- negative regulation of Rho protein signal transduction [IMP]
- negative regulation of cyclin-dependent protein serine/threonine kinase by cyclin degradation [IDA]
- positive regulation of cell proliferation [TAS]
- positive regulation of cytokinesis [IMP]
- positive regulation of mitotic metaphase/anaphase transition [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein monoubiquitination [IDA]
- protein polyubiquitination [IDA]
- protein ubiquitination [IDA]
- stem cell division [ISS]
- stress fiber assembly [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ANK1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Obscurin and KCTD6 regulate cullin-dependent small ankyrin-1 (sAnk1.5) protein turnover.
Protein turnover through cullin-3 is tightly regulated by posttranslational modifications, the COP9-signalosome and BTB/POZ-domain proteins that link cullin-3 to specific substrates for ubiquitylation. Here we report how KCTD6 represents a novel substrate adaptor for cullin-3, effectively regulating protein levels of the muscle ankyrin-1 isoform sAnk1.5.Binding of sAnk1.5 to KCTD6, and its subsequent turnover is regulated through posttranslational modification by nedd8, ... [more]
Throughput
- Low Throughput
Additional Notes
- likely ubiquitin conjugate
Curated By
- BioGRID