BAIT

CD5

LEU1, T1
CD5 molecule
GO Process (2)
GO Function (3)
GO Component (2)
Homo sapiens
PREY

LCK

IMD22, LSK, YT16, p56lck, pp58lck, RP4-675E8.4
LCK proto-oncogene, Src family tyrosine kinase
GO Process (30)
GO Function (14)
GO Component (7)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A new pathway of CD5 glycoprotein-mediated T cell inhibition dependent on inhibitory phosphorylation of Fyn kinase.

Bamberger M, Santos AM, Goncalves CM, Oliveira MI, James JR, Moreira A, Lozano F, Davis SJ, Carmo AM

Triggering of the T cell receptor initiates a signaling cascade resulting in the activation of the T cell. These signals are integrated alongside those resulting from the triggering of other receptors whose function is to modulate the overall response. CD5 is an immunotyrosine-based inhibition motif-bearing receptor that antagonizes the overt T cell receptor activation response by recruiting inhibitory intracellular mediators ... [more]

J. Biol. Chem. Sep. 02, 2011; 286(35);30324-36 [Pubmed: 21757751]

Throughput

  • Low Throughput

Curated By

  • BioGRID