BAIT

FLI1

EWSR2, SIC-1

Fli-1 proto-oncogene, ETS transcription factor

GO Process (4)

GO Function (3)

GO Component (1)

Gene Ontology Biological Process

cell differentiation [IBA]

hemostasis [TAS]

regulation of transcription from RNA polymerase II promoter [IBA]

transcription from RNA polymerase II promoter [IBA]

Gene Ontology Molecular Function

DNA binding [TAS]
sequence-specific DNA binding RNA polymerase II transcription factor activity [IBA]
sequence-specific DNA binding transcription factor activity [TAS]

Gene Ontology Cellular Component

CRISPR Database HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

PREY

UBE2I

C358B7.1, P18, UBC9, LA16c-358B7.1

ubiquitin-conjugating enzyme E2I

GO Process (8)

GO Function (9)

GO Component (6)

Gene Ontology Biological Process

cellular protein metabolic process [TAS]

cellular protein modification process [TAS]

negative regulation of transcription from RNA polymerase II promoter [IMP]

negative regulation of transcription, DNA-templated [IDA]

post-translational protein modification [TAS]

protein sumoylation [IDA, TAS]

protein ubiquitination [IBA]

ubiquitin-dependent protein catabolic process [TAS]

Gene Ontology Molecular Function

RING-like zinc finger domain binding [IPI]
SUMO transferase activity [IDA]
enzyme binding [IPI]
poly(A) RNA binding [IDA]
protein binding [IPI]
transcription factor binding [IPI]
ubiquitin protein ligase activity [IBA]
ubiquitin protein ligase binding [IBA]
ubiquitin-protein transferase activity [TAS]

Gene Ontology Cellular Component

cytoplasm [IDA]

nucleoplasm [TAS]

synaptonemal complex [TAS]

transferase complex [IDA]

CRISPR Database OMIM HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

FLI-1 functionally interacts with PIASxalpha, a member of the PIAS E3 SUMO ligase family.

van den Akker E, Ano S, Shih HM, Wang LC, Pironin M, Palvimo JJ, Kotaja N, Kirsh O, Dejean A, Ghysdael J

FLI-1 is a transcription factor of the ETS family that is involved in several developmental processes and that becomes oncogenic when overexpressed or mutated. As the functional regulators of FLI-1 are largely unknown, we performed a yeast two-hybrid screen with FLI-1 and identified the SUMO E3 ligase PIASxalpha/ARIP3 as a novel in vitro and in vivo binding partner of FLI-1. ... [more]

J. Biol. Chem. Nov. 11, 2005; 280(45);38035-46 [Pubmed: 16148010]

Throughput

Low Throughput

Curated By

BioGRID