An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Systematic survey of deubiquitinase localization identifies USP21 as a regulator of centrosome- and microtubule-associated functions.

Urbe S, Liu H, Hayes SD, Heride C, Rigden DJ, Clague MJ

Ubiquitination is a reversible modification that influences a broad range of physiological processes. There are approximately 90 deubiquitinases (DUBs) encoded in the human genome, of which 79 are predicted to have catalytic activity. We tagged 66 DUBs with green fluorescent protein and systematically surveyed their subcellular distribution, identifying enzymes specific to the nucleus, plasma membrane, and secretory and endocytic pathways. ... [more]

Mol. Biol. Cell Mar. 01, 2012; 23(6);1095-103 [Pubmed: 22298430]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

USP21

Gene Ontology Biological Process

Gene Ontology Molecular Function

NEDD8-specific protease activity [IDA]cysteine-type endopeptidase activity [IBA]cysteine-type peptidase activity [IMP, ISS]transcription coactivator activity [ISS]ubiquitin thiolesterase activity [IDA, ISS, TAS]ubiquitin-specific protease activity [IDA]

Gene Ontology Cellular Component

CKAP5