An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The Opitz syndrome gene product MID1 assembles a microtubule-associated ribonucleoprotein complex.

Aranda-Orgilles B, Trockenbacher A, Winter J, Aigner J, Koehler A, Jastrzebska E, Stahl J, Mueller EC, Otto A, Wanker EE, Schneider R, Schweiger S

Opitz BBB/G syndrome (OS) is a heterogenous malformation syndrome mainly characterised by hypertelorism and hypospadias. In addition, patients may present with several other defects of the ventral midline such as cleft lip and palate and congenital heart defects. The syndrome-causing gene encodes the X-linked E3 ubiquitin ligase MID1 that mediates ubiquitin-specific modification and degradation of the catalytic subunit of the ... [more]

Hum. Genet. Mar. 01, 2008; 123(2);163-76 [Pubmed: 18172692]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

MID1

Gene Ontology Biological Process

Gene Ontology Molecular Function

microtubule binding [IMP]phosphoprotein binding [IPI]protein heterodimerization activity [IDA]protein homodimerization activity [IDA]ubiquitin protein ligase binding [IPI]

Gene Ontology Cellular Component

HSP90AA1