BAIT
TUP1
AAR1, AER2, AMM1, CRT4, CYC9, FLK1, ROX4, SFL2, UMR7, chromatin-silencing transcriptional regulator TUP1, L000002391, YCR084C
General repressor of transcription; forms complex with Cyc8p, involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4, appears to enhance expression of some genes
GO Process (10)
GO Function (6)
GO Component (2)
Gene Ontology Biological Process
- histone exchange [IMP]
- negative regulation of dipeptide transport by negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of mating-type specific transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription from RNA polymerase II promoter by glucose [IMP]
- negative regulation of transcription from RNA polymerase II promoter during mitosis [IMP]
- nucleosome positioning [IDA]
- regulation of fatty acid biosynthetic process by regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of invasive growth in response to glucose limitation [IMP]
- regulation of response to DNA damage stimulus [IMP]
Gene Ontology Molecular Function- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [IDA, IMP]
- histone binding [IDA]
- histone deacetylase binding [IDA, IPI]
- mediator complex binding [IDA, IPI]
- phosphatidylinositol-3,5-bisphosphate binding [IDA]
- ubiquitin binding [IDA]
- RNA polymerase II transcription factor binding transcription factor activity involved in negative regulation of transcription [IDA, IMP]
- histone binding [IDA]
- histone deacetylase binding [IDA, IPI]
- mediator complex binding [IDA, IPI]
- phosphatidylinositol-3,5-bisphosphate binding [IDA]
- ubiquitin binding [IDA]
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
BIL1
EDO1, YOR304C-A
Protein that binds Bud6p and has a role in actin cable assembly; involved in the Bnr1p-dependent pathway of cable assembly; localizes to bud tip and bud neck
GO Process (1)
GO Function (0)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Coiled-Coil Networking Shapes Cell Molecular Machinery.
The highly abundant alpha-helical coiled-coil motif not only mediates crucial protein-protein interactions in the cell, but is also an attractive scaffold in synthetic biology and material science and a potential target for disease intervention. Therefore, a systematic understanding of the coiled-coil interactions at the organismal level would help unravel the full spectrum of the biological function of this interaction motif ... [more]
Mol. Biol. Cell Aug. 08, 2012; 0(0); [Pubmed: 22875988]
Throughput
- High Throughput
Additional Notes
- Interaction between cloned coiled-coil domains
Curated By
- BioGRID