An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Bifunctional apoptosis inhibitor (BAR) protects neurons from diverse cell death pathways.

Roth W, Kermer P, Krajewska M, Welsh K, Davis S, Krajewski S, Reed JC

The bifunctional apoptosis regulator (BAR) is a multidomain protein that was originally identified as an inhibitor of Bax-induced apoptosis. Immunoblot analysis of normal human tissues demonstrated high BAR expression in the brain, compared to low or absent expression in other organs. Immunohistochemical staining of human adult tissues revealed that the BAR protein is predominantly expressed by neurons in the central ... [more]

Cell Death Differ. Oct. 01, 2003; 10(10);1178-87 [Pubmed: 14502241]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

IFT57

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

BFAR