Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Structural requirements for interaction of peroxisomal targeting signal 2 and its receptor PEX7.

Kunze M, Neuberger G, Maurer-Stroh S, Ma J, Eck T, Braverman N, Schmid JA, Eisenhaber F, Berger J

The import of a subset of peroxisomal matrix proteins is mediated by the peroxisomal targeting signal 2 (PTS2). The results of our sequence and physical property analysis of known PTS2 signals and of a mutational study of the least characterized amino acids of a canonical PTS2 motif indicate that PTS2 forms an amphipathic helix accumulating all conserved residues on one ... [more]

J. Biol. Chem. Dec. 30, 2011; 286(52);45048-62 [Pubmed: 22057399]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

PEX7

Gene Ontology Biological Process

Gene Ontology Molecular Function

enzyme binding [IPI]peroxisome matrix targeting signal-2 binding [IDA]protein homodimerization activity [IDA]

Gene Ontology Cellular Component

ACAT1