Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly.

Blower MD, Nachury M, Heald R, Weis K

Centrosome-independent microtubule polymerization around chromosomes has been shown to require a local gradient of RanGTP, which discharges mitotic cargoes from the nuclear import receptor importin beta. Here, we have used an activity-based assay in Xenopus egg extracts to purify the mRNA export protein Rae1 as a spindle assembly factor regulated by this pathway. Rae1 is a microtubule-associated protein that binds ... [more]

Cell Apr. 22, 2005; 121(2);223-34 [Pubmed: 15851029]

Throughput

  • Low Throughput

Curated By

  • BioGRID