BAIT

TRIM72

MG53
tripartite motif containing 72, E3 ubiquitin protein ligase
GO Process (4)
GO Function (1)
GO Component (2)
Homo sapiens
PREY

MYH9

BDPLT6, DFNA17, EPSTS, FTNS, MHA, NMHC-II-A, NMMHC-IIA, NMMHCA, RP1-68O2.1
myosin, heavy chain 9, non-muscle
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Nonmuscle myosin IIA facilitates vesicle trafficking for MG53-mediated cell membrane repair.

Lin P, Zhu H, Cai C, Wang X, Cao C, Xiao R, Pan Z, Weisleder N, Takeshima H, Ma J

Repair of injury to the plasma membrane is an essential mechanism for maintenance of cellular homeostasis and integrity that involves coordinated movement of intracellular vesicles to membrane injury sites to facilitate patch formation. We have previously identified MG53 as an essential component of the cell membrane repair machinery. In order for MG53 and intracellular vesicles to translocate to membrane injury ... [more]

FASEB J. May. 01, 2012; 26(5);1875-83 [Pubmed: 22253476]

Throughput

  • Low Throughput

Curated By

  • BioGRID