An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.

Publication

Polo-like kinase 1 regulates Nlp, a centrosome protein involved in microtubule nucleation.

Casenghi M, Meraldi P, Weinhart U, Duncan PI, Koerner R, Nigg EA

In animal cells, most microtubules are nucleated at centrosomes. At the onset of mitosis, centrosomes undergo a structural reorganization, termed maturation, which leads to increased microtubule nucleation activity. Centrosome maturation is regulated by several kinases, including Polo-like kinase 1 (Plk1). Here, we identify a centrosomal Plk1 substrate, termed Nlp (ninein-like protein), whose properties suggest an important role in microtubule organization. ... [more]

Dev. Cell Jul. 01, 2003; 5(1);113-25 [Pubmed: 12852856]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NINL PLK1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Casenghi M (2003)	Low	-	BioGRID	-
NINL PLK1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Gupta GD (2015)	High	-	BioGRID	1432821
NINL PLK1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Redwine WB (2017)	High	-	BioGRID	-
PLK1 NINL	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Casenghi M (2003)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

PLK1

Gene Ontology Biological Process

Gene Ontology Molecular Function

anaphase-promoting complex binding [IPI]kinase activity [TAS]microtubule binding [IDA]protein binding [IPI]protein kinase activity [IDA]protein kinase binding [IPI]protein serine/threonine kinase activity [EXP, IDA, IMP]

Gene Ontology Cellular Component

NINL