AGO2
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- Notch signaling pathway [TAS]
- RNA phosphodiester bond hydrolysis, endonucleolytic [EXP, IDA]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- gene expression [TAS]
- gene silencing by RNA [ISS]
- innate immune response [TAS]
- mRNA cleavage involved in gene silencing by miRNA [IDA, IMP]
- negative regulation of translation involved in gene silencing by miRNA [IDA, IMP]
- negative regulation of translational initiation [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [ISS]
- positive regulation of nuclear-transcribed mRNA poly(A) tail shortening [ISS]
- pre-miRNA processing [IDA]
- translation [NAS]
- translational initiation [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
EIF4EBP1
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IMP]
- TOR signaling [IDA]
- cellular protein metabolic process [TAS]
- gene expression [TAS]
- insulin receptor signaling pathway [TAS]
- negative regulation of translational initiation [IDA]
- positive regulation of mitotic cell cycle [IMP]
- translation [TAS]
- translational initiation [TAS]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression.
Gain-of-function mutations in leucine-rich repeat kinase 2 (LRRK2) cause familial as well as sporadic Parkinson's disease characterized by age-dependent degeneration of dopaminergic neurons. The molecular mechanism of LRRK2 action is not known. Here we show that LRRK2 interacts with the microRNA (miRNA) pathway to regulate protein synthesis. Drosophila e2f1 and dp messenger RNAs are translationally repressed by let-7 and miR-184*, ... [more]
Throughput
- Low Throughput
Additional Notes
- figure S10.
Curated By
- BioGRID