PREY

SERPINF2

A2AP, AAP, ALPHA-2-PI, API, PLI
serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Mannose trimming targets mutant alpha(2)-plasmin inhibitor for degradation by the proteasome.

Chung DH, Ohashi K, Watanabe M, Miyasaka N, Hirosawa S

We have previously characterized the molecular and cellular mechanisms of alpha(2)-plasmin inhibitor (alpha(2)PI) deficiency. The mutant alpha(2)PI-Nara and alpha(2)PI-Okinawa proteins were found to be retained and degraded in cells stably expressing these mutant forms of alpha(2)PI. Degradation of the two mutant alpha(2)PI proteins, mediated by proteasomes, occurred after a lag time of 1.5 h during which glucose trimming took place. ... [more]

J. Biol. Chem. Feb. 18, 2000; 275(7);4981-7 [Pubmed: 10671537]

Throughput

  • Low Throughput

Curated By

  • BioGRID