BAIT
SMAD3
HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3
SMAD family member 3
GO Process (35)
GO Function (24)
GO Component (10)
Gene Ontology Biological Process
- SMAD protein complex assembly [IDA]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- activin receptor signaling pathway [IMP]
- cell cycle arrest [IMP]
- cell-cell junction organization [IMP]
- evasion or tolerance of host defenses by virus [IDA]
- extrinsic apoptotic signaling pathway [IMP]
- gene expression [TAS]
- immune response [IMP]
- intracellular signal transduction [IDA]
- negative regulation of cell growth [IDA]
- negative regulation of mitotic cell cycle [IMP]
- negative regulation of protein catabolic process [IMP]
- negative regulation of protein phosphorylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IMP, TAS]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- nodal signaling pathway [IMP]
- positive regulation of canonical Wnt signaling pathway [IMP]
- positive regulation of catenin import into nucleus [IMP]
- positive regulation of epithelial to mesenchymal transition [IDA, IMP]
- positive regulation of gene expression [IDA]
- positive regulation of transcription factor import into nucleus [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP, TAS]
- positive regulation of transcription, DNA-templated [IDA]
- primary miRNA processing [TAS]
- protein stabilization [IMP]
- regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of transforming growth factor beta receptor signaling pathway [IMP]
- regulation of transforming growth factor beta2 production [IMP]
- response to hypoxia [IMP]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [IDA, TAS]
- transport [IDA]
- wound healing [TAS]
Gene Ontology Molecular Function- R-SMAD binding [IPI]
- RNA polymerase II activating transcription factor binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [IDA]
- bHLH transcription factor binding [IPI]
- beta-catenin binding [IPI]
- co-SMAD binding [IPI]
- core promoter proximal region sequence-specific DNA binding [IDA]
- enhancer binding [IC]
- identical protein binding [IPI]
- phosphatase binding [IPI]
- protein binding [IPI]
- protein binding transcription factor activity [IDA]
- protein homodimerization activity [IPI]
- protein kinase binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor binding [IPI]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [IDA]
- ubiquitin binding [IDA]
- ubiquitin protein ligase binding [IPI]
- zinc ion binding [IDA]
- R-SMAD binding [IPI]
- RNA polymerase II activating transcription factor binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [IDA]
- bHLH transcription factor binding [IPI]
- beta-catenin binding [IPI]
- co-SMAD binding [IPI]
- core promoter proximal region sequence-specific DNA binding [IDA]
- enhancer binding [IC]
- identical protein binding [IPI]
- phosphatase binding [IPI]
- protein binding [IPI]
- protein binding transcription factor activity [IDA]
- protein homodimerization activity [IPI]
- protein kinase binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor binding [IPI]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [IDA]
- ubiquitin binding [IDA]
- ubiquitin protein ligase binding [IPI]
- zinc ion binding [IDA]
Gene Ontology Cellular Component
Homo sapiens
PREY
SMAD1
BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1
SMAD family member 1
GO Process (10)
GO Function (10)
GO Component (8)
Gene Ontology Biological Process
- BMP signaling pathway [IDA, IMP, ISS, TAS]
- SMAD protein complex assembly [IDA]
- cellular response to BMP stimulus [ISS]
- embryonic pattern specification [ISS]
- positive regulation of gene expression [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IC, IDA]
- positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus [ISS]
- primary miRNA processing [TAS]
- signal transduction [NAS]
- transforming growth factor beta receptor signaling pathway [NAS]
Gene Ontology Molecular Function- I-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- co-SMAD binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- receptor signaling protein activity [NAS]
- sequence-specific DNA binding transcription factor activity [IDA]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [TAS]
- I-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- co-SMAD binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- receptor signaling protein activity [NAS]
- sequence-specific DNA binding transcription factor activity [IDA]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [TAS]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Identification of novel Smad2 and Smad3 associated proteins in response to TGF-beta1.
Transforming growth factor-beta 1 (TGF-beta1) is an important growth inhibitor of epithelial cells and insensitivity to this cytokine results in uncontrolled cell proliferation and can contribute to tumorigenesis. TGF-beta1 signals through the TGF-beta type I and type II receptors, and activates the Smad pathway via phosphorylation of Smad2 and Smad3. Since little is known about the selective activation of Smad2 ... [more]
J. Cell. Biochem. Oct. 01, 2008; 105(2);596-611 [Pubmed: 18729074]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SMAD1 SMAD3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
| SMAD3 SMAD1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID