BAIT
SMAD1
BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1
SMAD family member 1
GO Process (10)
GO Function (10)
GO Component (8)
Gene Ontology Biological Process
- BMP signaling pathway [IDA, IMP, ISS, TAS]
- SMAD protein complex assembly [IDA]
- cellular response to BMP stimulus [ISS]
- embryonic pattern specification [ISS]
- positive regulation of gene expression [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IC, IDA]
- positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus [ISS]
- primary miRNA processing [TAS]
- signal transduction [NAS]
- transforming growth factor beta receptor signaling pathway [NAS]
Gene Ontology Molecular Function- I-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- co-SMAD binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- receptor signaling protein activity [NAS]
- sequence-specific DNA binding transcription factor activity [IDA]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [TAS]
- I-SMAD binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- co-SMAD binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- receptor signaling protein activity [NAS]
- sequence-specific DNA binding transcription factor activity [IDA]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [TAS]
Gene Ontology Cellular Component
Homo sapiens
PREY
SMAD3
HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3
SMAD family member 3
GO Process (35)
GO Function (24)
GO Component (10)
Gene Ontology Biological Process
- SMAD protein complex assembly [IDA]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- activin receptor signaling pathway [IMP]
- cell cycle arrest [IMP]
- cell-cell junction organization [IMP]
- evasion or tolerance of host defenses by virus [IDA]
- extrinsic apoptotic signaling pathway [IMP]
- gene expression [TAS]
- immune response [IMP]
- intracellular signal transduction [IDA]
- negative regulation of cell growth [IDA]
- negative regulation of mitotic cell cycle [IMP]
- negative regulation of protein catabolic process [IMP]
- negative regulation of protein phosphorylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IMP, TAS]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- nodal signaling pathway [IMP]
- positive regulation of canonical Wnt signaling pathway [IMP]
- positive regulation of catenin import into nucleus [IMP]
- positive regulation of epithelial to mesenchymal transition [IDA, IMP]
- positive regulation of gene expression [IDA]
- positive regulation of transcription factor import into nucleus [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP, TAS]
- positive regulation of transcription, DNA-templated [IDA]
- primary miRNA processing [TAS]
- protein stabilization [IMP]
- regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of transforming growth factor beta receptor signaling pathway [IMP]
- regulation of transforming growth factor beta2 production [IMP]
- response to hypoxia [IMP]
- transcription initiation from RNA polymerase II promoter [TAS]
- transcription, DNA-templated [TAS]
- transforming growth factor beta receptor signaling pathway [IDA, TAS]
- transport [IDA]
- wound healing [TAS]
Gene Ontology Molecular Function- R-SMAD binding [IPI]
- RNA polymerase II activating transcription factor binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [IDA]
- bHLH transcription factor binding [IPI]
- beta-catenin binding [IPI]
- co-SMAD binding [IPI]
- core promoter proximal region sequence-specific DNA binding [IDA]
- enhancer binding [IC]
- identical protein binding [IPI]
- phosphatase binding [IPI]
- protein binding [IPI]
- protein binding transcription factor activity [IDA]
- protein homodimerization activity [IPI]
- protein kinase binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor binding [IPI]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [IDA]
- ubiquitin binding [IDA]
- ubiquitin protein ligase binding [IPI]
- zinc ion binding [IDA]
- R-SMAD binding [IPI]
- RNA polymerase II activating transcription factor binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity [IDA]
- bHLH transcription factor binding [IPI]
- beta-catenin binding [IPI]
- co-SMAD binding [IPI]
- core promoter proximal region sequence-specific DNA binding [IDA]
- enhancer binding [IC]
- identical protein binding [IPI]
- phosphatase binding [IPI]
- protein binding [IPI]
- protein binding transcription factor activity [IDA]
- protein homodimerization activity [IPI]
- protein kinase binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription factor binding [IPI]
- transcription regulatory region DNA binding [IDA]
- transforming growth factor beta receptor binding [IPI]
- transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity [IDA]
- ubiquitin binding [IDA]
- ubiquitin protein ligase binding [IPI]
- zinc ion binding [IDA]
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Heteromeric and homomeric interactions correlate with signaling activity and functional cooperativity of Smad3 and Smad4/DPC4.
Homologs of Drosophila Mad function as downstream mediators of the receptors for transforming growth factor beta (TGF-beta)-related factors. Two homologs, the receptor-associated Smad3 and the tumor suppressor Smad4/DPC4, synergize to induce ligand-independent TGF-beta activities and are essential mediators of the natural TGF-beta response. We now show that Smad3 and Smad4 associate in homomeric and heteromeric interactions, as assessed by yeast ... [more]
Mol. Cell. Biol. May. 01, 1997; 17(5);2521-8 [Pubmed: 9111321]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SMAD3 SMAD1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| SMAD3 SMAD1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID