Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

[Identification of the proteins interacting with neuroprotective peptide humanin in a yeast two-hybrid system].

Maksimov VV, Arman IP, Tarantul VZ

Humanine is a human neuroprotective peptide with a wide action spectrum. To analyze molecular mechanisms of humanin functioning, a search for proteins interacting with this peptide was conducted using yeast two-hybrid system. Screening of human fetal brain cDNA library identified seven proteins with different functions that specifically interacted with humanin. ... [more]

Genetika Feb. 01, 2006; 42(2);274-7 [Pubmed: 16583711]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

MTRNR2L1

EEF1A1

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTP binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]protein kinase binding [IPI]translation elongation factor activity [TAS]

Gene Ontology Cellular Component

Two-hybrid

Publication

[Identification of the proteins interacting with neuroprotective peptide humanin in a yeast two-hybrid system].

Throughput

Curated By

GTP binding [TAS]
poly(A) RNA binding [IDA]
protein binding [IPI]
protein kinase binding [IPI]
translation elongation factor activity [TAS]