PREY

ASIC2

ACIC2, Accn1, BNC1, BNaC1, BNaC1a, Mdeg, RP23-397J5.1
acid-sensing (proton-gated) ion channel 2
Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

ASIC2 subunits target acid-sensing ion channels to the synapse via an association with PSD-95.

Zha XM, Costa V, Harding AM, Reznikov L, Benson CJ, Welsh MJ

Acid-sensing ion channel-1a (ASIC1a) mediates H(+)-gated current to influence normal brain physiology and impact several models of disease. Although ASIC2 subunits are widely expressed in brain and modulate ASIC1a current, their function remains poorly understood. We identified ASIC2a in dendrites, dendritic spines, and brain synaptosomes. This localization largely relied on ASIC2a binding to PSD-95 and matched that of ASIC1a, which ... [more]

J. Neurosci. Jul. 01, 2009; 29(26);8438-46 [Pubmed: 19571134]

Throughput

  • Low Throughput

Additional Notes

  • Myc-ASIC2a transgenic (TG) mice

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ASIC2 ASIC1
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-

Curated By

  • BioGRID