PDX1
Gene Ontology Biological Process
- cell differentiation [IMP, ISO]
- detection of glucose [ISO]
- digestive tract development [IMP]
- endocrine pancreas development [IMP]
- exocrine pancreas development [IMP]
- glucose homeostasis [IGI, IMP, ISO]
- glucose metabolic process [IDA]
- insulin secretion [ISO]
- liver development [IMP]
- morphogenesis of embryonic epithelium [IMP]
- negative regulation of cell proliferation [IMP]
- negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [ISO]
- negative regulation of type B pancreatic cell apoptotic process [IMP]
- nitric oxide mediated signal transduction [ISO]
- pancreas development [IMP]
- positive regulation of DNA binding [ISO]
- positive regulation of cell death [ISO]
- positive regulation of cell proliferation [IMP, ISO]
- positive regulation of insulin secretion [ISO]
- positive regulation of insulin secretion involved in cellular response to glucose stimulus [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, ISO]
- positive regulation of transcription, DNA-templated [IDA, ISO]
- regulation of cell proliferation [IDA]
- regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- response to glucose [ISO]
- smoothened signaling pathway [ISO]
- stem cell differentiation [ISO]
- transcription from RNA polymerase II promoter [ISO]
- transcription, DNA-templated [ISO]
- type B pancreatic cell differentiation [ISO]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [ISO]
- chromatin binding [IDA]
- core promoter sequence-specific DNA binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein heterodimerization activity [ISO]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription factor binding [ISO]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [ISO]
- chromatin binding [IDA]
- core promoter sequence-specific DNA binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein heterodimerization activity [ISO]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription factor binding [ISO]
Gene Ontology Cellular Component
PCIF1
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Two conserved domains in PCIF1 mediate interaction with pancreatic transcription factor PDX-1.
PCIF1 is a TRAF and POZ domain containing nuclear factor that interacts with and inhibits transactivation of pancreatic homeodomain transcription factor PDX-1. Here, we demonstrate interaction of endogenous PDX-1 and PCIF1 in MIN6 insulinoma cells. Within PCIF1, the TRAF and POZ domains are both required for physical and functional interaction with the C-terminus of PDX-1, whereas the C-terminal domain of ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PDX1 PCIF1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PDX1 PCIF1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| PDX1 PCIF1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| PDX1 PCIF1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID