DMBX1
Gene Ontology Biological Process
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- DNA binding [IDA]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
Gene Ontology Cellular Component
OTX2
Gene Ontology Biological Process
- anatomical structure development [IGI]
- anterior/posterior pattern specification [IGI, IMP]
- axon guidance [ISO]
- cell differentiation [IMP]
- cell fate commitment [IMP]
- cell fate specification [IMP]
- central nervous system development [IMP]
- diencephalon morphogenesis [IGI]
- dorsal/ventral pattern formation [IMP]
- endoderm development [IGI]
- eye photoreceptor cell fate commitment [IMP]
- forebrain development [IGI, IMP]
- inner ear morphogenesis [IGI]
- metencephalon development [IGI]
- midbrain development [IGI, IMP]
- neuron differentiation [IMP]
- neuron fate commitment [IMP]
- neuron fate determination [IMP]
- organ morphogenesis [IMP]
- positive regulation of embryonic development [IMP]
- positive regulation of gastrulation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IGI, ISO]
- positive regulation of transcription, DNA-templated [IMP]
- primitive streak formation [IMP]
- protein complex assembly [ISO]
- regulation of transcription from RNA polymerase II promoter [IDA]
- somite rostral/caudal axis specification [IMP]
- transcription from RNA polymerase II promoter [ISO]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
Gene Ontology Cellular Component
- cytoplasm [IDA]
- growth cone [ISO]
- nucleus [IC, IDA]
- protein complex [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Functional analysis of transcriptional repressor Otx3/Dmbx1.
Otx3/Dmbx1 is a member of paired class homeodomain transcription factors. In this study, we found that Otx3/Dmbx1 represses the Otx2-mediated transactivation by forming heterodimer with Otx2 on the P3C (TAATCCGATTA) sequence in vitro. The 156 amino acid region (residues 1-156) of Otx3/Dmbx1 is required for its repressor activity, and interacts directly with Otx2. Co-localization of Otx3/Dmbx1 and Otx2 in brain ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| OTX2 DMBX1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID