BAIT
DUN1
serine/threonine protein kinase DUN1, L000000531, YDL101C
Cell-cycle checkpoint serine-threonine kinase; required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; Mec1p and Dun1p function in same pathway to regulate both dNTP pools and telomere length; also regulates postreplicative DNA repair
GO Process (3)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Saccharomyces cerevisiae (S288c)
PREY
MET32
L000003470, YDR253C
Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; lack of such a loop for MET31 may account for the differential actions of Met32p and Met31p; MET32 has a paralog, MET31, that arose from the whole genome duplication
GO Process (5)
GO Function (4)
GO Component (2)
Gene Ontology Biological Process
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of mitotic cell cycle [IGI, IMP]
- regulation of sulfur amino acid metabolic process [IDA, IGI, IMP]
- regulation of transcription from RNA polymerase II promoter [IDA, IGI]
Gene Ontology Molecular Function- RNA polymerase II activating transcription factor binding [IDA, IPI]
- core promoter proximal region sequence-specific DNA binding [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity [IDA, IGI, IPI]
- RNA polymerase II activating transcription factor binding [IDA, IPI]
- core promoter proximal region sequence-specific DNA binding [IDA]
- sequence-specific DNA binding [IDA]
- sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity [IDA, IGI, IPI]
Saccharomyces cerevisiae (S288c)
Dosage Lethality
A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.
Publication
Functional analysis with a barcoder yeast gene overexpression system.
Systematic analysis of gene overexpression phenotypes provides an insight into gene function, enzyme targets, and biological pathways. Here, we describe a novel functional genomics platform that enables a highly parallel and systematic assessment of overexpression phenotypes in pooled cultures. First, we constructed a genome-level collection of ~5100 yeast barcoder strains, each of which carries a unique barcode, enabling pooled fitness ... [more]
G3 (Bethesda) Oct. 01, 2012; 2(10);1279-89 [Pubmed: 23050238]
Throughput
- High Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Additional Notes
- conditional SDL hits in 0.001% MMS (5 Generations of treatment)
Curated By
- BioGRID